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A “latch” module (consisting of the β-hairpin loop that emanates within the OB area) occupies the foremost groove and completes the circumferential clamp via contacts in between the suggestion of the loop and also the surface area with the NTase area. The clamp-closing contacts are positioned at “six o'clock” to the DNA circumference (denoted because of the purple arrow
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This situation invites the design of C-2-substituted analogs of adenosine nucleotides and NAD+ as selective inhibitors of LigA. In truth, it was documented recently that 2-methylthio-ATP is often a potent inhibitor of your adenylylation reaction of E. coli
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The motion involves a rigid entire body rotation in regards to the hinge segment connecting the NTase and OB domains (indicated by the arrow
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